<div>Hi,</div>
<div>
<p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US"><font face="Calibri" size="3">If you cannot solve the problem finally, a commercial company may helpful to you. </font><a href="http://www.genscript.com/custom_protein_purification_characterization.html"><font face="Calibri" color="#800080" size="3">http://www.genscript.com/custom_protein_purification_characterization.html</font></a><font face="Calibri" size="3"> </font></span></p>
<br><br></div>
<div class="gmail_quote">On Thu, May 15, 2008 at 1:07 AM, <<a href="mailto:yeast-request@oat.bio.indiana.edu">yeast-request@oat.bio.indiana.edu</a>> wrote:<br>
<blockquote class="gmail_quote" style="PADDING-LEFT: 1ex; MARGIN: 0px 0px 0px 0.8ex; BORDER-LEFT: #ccc 1px solid">Send Yeast mailing list submissions to<br> <a href="mailto:yeast@net.bio.net">yeast@net.bio.net</a><br>
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than "Re: Contents of Yeast digest..."<br><br><br>Today's Topics:<br><br> 1. (no subject) (<a href="mailto:jkellosa@mappi.helsinki.fi">jkellosa@mappi.helsinki.fi</a>)<br><br><br>----------------------------------------------------------------------<br>
<br>Message: 1<br>Date: Wed, 14 May 2008 14:10:19 +0300<br>From: <a href="mailto:jkellosa@mappi.helsinki.fi">jkellosa@mappi.helsinki.fi</a><br>Subject: [Yeast] (no subject)<br>To: <a href="mailto:yeast@magpie.bio.indiana.edu">yeast@magpie.bio.indiana.edu</a><br>
Message-ID: <<a href="mailto:1210763419.482ac89b7d908@webmail.helsinki.fi">1210763419.482ac89b7d908@webmail.helsinki.fi</a>><br>Content-Type: text/plain; charset=us-ascii<br><br>Dear all,<br><br>A lab technician who I work with got both pure protein and wanted protein<br>
with two contaminating bands, both of which molecular weight is higher than<br>the molecular weight of the wanted protein, from different batches of<br>protein purification. The molecular weights of the contaminating bands are<br>
not high enough to account for different oligomerization states of the<br>wanted protein.<br><br>The two contaminating protein bands hang on really tightly with the wanted<br>protein and we have not been able to get rid of them.<br>
<br>I dont know what is the difference between purifications which produce pure<br>protein and the purifications which don't, but I suspect that at least<br>there has been differences at how long the cells have been growing since<br>
they were harvested.<br><br>This leads me to my question that I want to ask from you:<br><br>Have you ever had problems with contaminating proteins (stable<br>proteins/chaperons hanging along, modifications of the wanted protein ?)<br>
when S.cerevisae cells have been in a certain growth phase ?<br><br>-Juho Kellosalo<br><br><br><br><br>------------------------------<br><br>_______________________________________________<br>Yeast mailing list<br><a href="mailto:Yeast@net.bio.net">Yeast@net.bio.net</a><br>
<a href="http://www.bio.net/biomail/listinfo/yeast" target="_blank">http://www.bio.net/biomail/listinfo/yeast</a><br><br>End of Yeast Digest, Vol 36, Issue 7<br>************************************<br></blockquote></div><br>
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