Hello! this is my first post to this listserv, so please pardon me if I am a bit overly verbose... <br><br>I was updating my sequence files & vector maps recently for various plasmids and I noticed something that might be of interest to anyone using the 'pREP' series vectors in S.pombe. <br>
<br><a href="http://www.ncbi.nlm.nih.gov/pubmed/12597277">Watanabe T et.al. (2002)</a> published a report that indicated there were a number of poly(A) bearing RNAs without long open reading frames expressed in S.pombe (so called 'prl' genes). One of them, prl10 (Genbank <a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=26006032">AB084822</a>), is a 692-bp transcript that could produce a small 40 a.a. protein. What was interesting to me was that prl10 sits immediately upstream of nmt1. Since the nmt1 promoter region is used in the pREP series plasmids, thus so is the entire prl10 gene. Whether or not the prl10 transcript is expressed from pREP vectors is not known (to me).<br>
<br>So I was curious: has anyone else noticed this before? And is it known whether or not pREP series plasmids express the prl10 transcript? And finally, is it known if there is a phenotype associated with a prl10 deletion strain? Does anyone have a hint as to what the function of this RNA may be?<br clear="all">
<br>Jonathan Jacobs, Ph.D.<br>NICHD / NIH<br>lab: (301) 402-1155<br>cell: (240) 447-4039<br><br><a href="http://www.linkedin.com/in/jonathanjacobs">http://www.linkedin.com/in/jonathanjacobs</a><br><a href="http://www.citeulike.org/user/jonathanjacobs">http://www.citeulike.org/user/jonathanjacobs</a><br>
