<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<HTML><HEAD>
<META http-equiv=Content-Type content="text/html; charset=iso-8859-1">
<META content="MSHTML 6.00.6001.18203" name=GENERATOR>
<STYLE></STYLE>
</HEAD>
<BODY bgColor=#ffffff>
<DIV><FONT face=Arial size=2>Hi, there,</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Does anybody know the casue of a much more slower
growth rate of transformans? we introduced a 5kb heterologous ABC
transporter gene in to Saccharomyces cerevisiae single mutant for a
complementation experiment( integration vector, URA3 selection), the
transformants did show up on selective medium but stay as a very little tiny
colonies even after incubation for up to one week. any trouble shooting tips on
this issue? </FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Thanks in advance.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Ye</FONT></DIV></BODY></HTML>
Send comments to us at
archive@iubio.bio.indiana.edu
