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<div><font style="BACKGROUND-COLOR: #c0c0c0">Hi,</font></div>
<div><font style="BACKGROUND-COLOR: #c0c0c0"></font> </div>
<div><font style="BACKGROUND-COLOR: #c0c0c0">I am purifying a protein that is being secreted to culture medium, however, I encounter significant amount of cytosolic proteins in the supe. I guess these are resulted from cell lysis. If it is true, I'd like to monitor how much yeast cell lysis is going on during exponential cell growth. </font></div>
<div><font style="BACKGROUND-COLOR: #c0c0c0"></font> </div>
<div><font style="BACKGROUND-COLOR: #c0c0c0">Please give me some advise/comments for markers, methods and references. My email is <a href="mailto:jinyohan@gmail.com">jinyohan@gmail.com</a></font></div>
<div><font style="BACKGROUND-COLOR: #c0c0c0"></font> </div>
<div><font style="BACKGROUND-COLOR: #c0c0c0">Thank you so much, in advance.</font></div>
<div><font style="BACKGROUND-COLOR: #c0c0c0"></font> </div>
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<div><font style="BACKGROUND-COLOR: #c0c0c0">Yohan Jin</font></div></font></div><br>
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