APPEL at UONEURO.UOREGON.EDU
Tue Nov 15 11:59:14 EST 1994
I favor the method of Chomczynski and Sacchi (1987, Analytical Biochemistry,
162: 156-159) for isolation of RNA isolation from all stages of zebrafish
embryos. It's fast, reliable, and gives good quality RNA. If you plan to do
RT-PCR, you should be careful that the acid phenol extraction removes all
chromosomal DNA. You may have to treat your RNA with a RNase-free DNase.
I've never done this on eggs, so I don't know yield. However, from 3 hr old
embryos, I've recovered 160 ng total RNA per embryo. I've also used a kit
from Clontech, the Micro-Scale RNA Separator. It's the same method as
Let me know if you have more questions.
Inst. of Neuroscience
University of Oregon
appel at uoneuro.uoregon.edu
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