early embryonic death
gdoerre at mail2.sas.upenn.edu
Mon Feb 20 17:39:21 EST 1995
BREITBART at PHENIX.TCH.HARVARD.EDU wrote:
: From time to time, we find a very high (up to 50%) death rate from wild-type
: crosses in the first few hours after laying. We are told that this may res
: from lack of (i.e. incomplete) fertilization. Two questions:
: 1. Is there a method to determine definitively that fertilization is
: the problem?
well, sometimes we see less fertilized embryos than normally; generally
it's obvious when the embryos (just oocytes, i suppose) remain at the one
cell stage til they die a few hours later. if your males are old and
can't get around as much anymore, this may be a problem
: 2. What are other likely causes of such high embryo attrition?
otherwise: how old are these fish? after about two years, the embryos
we get from certain females deteriorate rapidly, although they appear to
be dividing fine for a few hours. don't have any explanation other than
old age, though.
|\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/|gdoerre at sas.upenn.edu
More information about the Zbrafish