(none)

brent butts bbutts at KRUNCHER.PTLOMA.EDU
Thu Feb 29 19:40:32 EST 1996


To Whom It May Concern:

        I am trying to put together a developmental lab for the rest of my
Developmental Class here at Point Loma Nazarene College, (San Diego), and am
interested in doing something with PCR and Zebrafish.
        I would like to do some type of lab where the other students would
do PCR from different stages of Zebrafish embryos, in order to amplify up a
gene that would be present in one stage but not the other.
        I have DNA available from the gastrula stage (6-9 hr.), neurula
stage (9-16 hr.), post somitigenesis stage (20-28 hr.), and adult DNA.
        Therefore, I was wondering if anyone could tell me of any genes that
would be expressed in any one of these stages but not in any others.
Moreover, I can design the primers, but in that case, I would also need to
know the sequence of any of these genes anyone can suggest, or the genbank
number of the gene so I can get the sequence. Thank you very much. I am not
on the "fishnet", so any answers should be sent to :
        bbutts at kruncher.ptloma.edu
 Thanks for your help.





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