Pigmentless embryos???

Richard Vogt vogt
Tue Jul 16 17:59:11 EST 1996

I need to make zebrafish without eye pigment to check for expression of a gene
in the eye.  Does anyone have experience using PTU?  I've worked with PTU
enough to know it is pretty insoluble.  I notice the Zeb Book says 0.003% PTU
in Hanks.  Do you just put in enought PTU to pretend it is 0.003% or is there
some trick to getting it to go into solution?  Any advice is welcome.  Thanks

Dick Vogt

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