Summary for fry anesthesia
ylin at ACPUB.DUKE.EDU
Tue Jun 4 14:04:32 EST 1996
Thanks for all of the responses that I received concerning my question on
the best way to anesthetize fry. Some of the responses weren't posted to
the list, so here's a short summary of what I got:
1. tricane (with its may aliases) solution at 1/2ppt, 0.05mg/mL, or 0.2%
(I haven't figured out how these different concentrations compare).
4. chlorobutaol (2.5-5.0mM)
I tried the 0.05mg/mL tricane solution, which put the fry under rather
well. Some of the fry still twitched a bit. My biggest problem was
trying to revive the fish afterwards. Most of the fish came back ok, but
I lost a couple. I'm not sure, however, if the cause of the deaths was
the tricane, or being under the scope for a while, or damage caused to
them in their transferring from solutions.
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