Autofluorescence in zebrafish
ylin at ACPUB.DUKE.EDU
Sat Nov 23 21:20:46 EST 1996
I was wondering if anyone has taken a close look at the auto-fluorescence
that comes from zfish fry? I was trying to get a more rigorous and
quantitative sense of what auto-fluor occurs in zfish fry.
(The equiptment that I'm using is a Hg epi-fluor illuminator, using a
FITC (exitiation filter: 455-500nm; emission filter: 510-560nm) filter
With 24hr fry, I see a fair amount of auto-fluor, but that isn't as
critical, since the bulk of the fry isn't obscured by it. However, going
down to 40x, I saw a fair number of small, rather bright spots of green.
Does anyone have any idea of what these are or could be?
At 48hrs, the autofluor gets rather bright. Rather interesting patterns
arise from the pigmentation that's developing. It's rather difficult to
optically section the embryo (probably because of the pigmenting?) and
with the bright autofluor, I wasn't able to find any of the spots of
green that I saw with the 24hr fry.
I notice that confocal microscopy is rather good at taking out the
autofluor (probably because of the very tight excitation wavelength and
the diffuseness of autofluor at a single plane), but I wonder if those
distinct spots that I saw with the 24hr fry would appear on confocal
Has anyone observed the same things, and/or have suggestions about
dealing with it?
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