A serumless culture
tmiyake at IS.DAL.CA
Tue Apr 29 18:16:54 EST 1997
I have developed an organ culture system to study cellular and molecular
mechanisms that regulate differentiation and morphogenesis of the
skeleton in zebrafish and Japanese Medaka (Miyake and Hall., J. Exp.
Zool., 268: 22-43, 1994). I found that M199 and Leibovitz's L-15 are
suitable to do organ culture with FBS. Horse serum was found to be toxic
to tissues. The system I developed was one to place tissues in a medium
submerged. An air-CO2 interface system for chicks and mice did not work
I am just wondering if there are previous studies that used a serumless
culture system in zebrafish. It does not matter if it was developed for
another species of fishes. Serum factors may complicate analyses of in
vitro studies on skeletal development.
Dr. Tsutomu (Tom) Miyake, Ph.D.
Department of Biology
1355 Oxford Street
Life Sciences Center
Halifax, Nova Scotia
CANADA B3H 4J1
TEL/FAX: 902-429-8670 (home)
E-MAIL: tmiyake at is.dal.ca
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