A serumless culture system

Tsutomu T Miyake tmiyake at IS.DAL.CA
Tue Apr 29 16:29:05 EST 1997


Dear Madam/Sir

I have developed an organ culture system to study cellular and molecualr
mechanisms that regulate differentiation and morphogenesis of the skeleton
in zebrafihs and Japanese Medaka (Miyake and Hall, 1994, J. Exp. Zool.,
268: 22-43).  The system was based on submerged culture of tissues by
using M199 or Leibovitz's L-15 medium with FBS.  The horse serum was found to be
toxic to skeletal tissues.  I am wondering if there are previous studies
that used a serumless organ or cell culture system in zebrafish.  It does
not matter if it was originally developed for another species or groups of
fishes.  

Dr. Tom Miyake
Department of Biology
Dalhousie University
1355 Oxford Street
Life Science Building
Halifax, Nova Scotia
Canada   B3H 4J1
TEL: 902-494-3335
FAX: 902-494-3736
E-MAIL: tmiyake at is.da.ca



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