Fix for zfish & GFP?
ylin at ACPUB.DUKE.EDU
Fri Feb 21 15:23:07 EST 1997
Thanks to all who have replied!
I still have my samples sitting in 4% PF (since 2/17, at 4degC), so I
pulled them out and took a look. I've taken a few pictures, and
have them on the same web site:
I've tried to reproduce the conditions under which I had taken the other
pictures (except for the left, 4-day-fix picture, which is at half the
exposure at which the other ones were taken [whoops! :/ ]).
After 4 days, you can still see the glowing muscle fiber, although it's
rather faint. I've put another pre-fix picture on the page (the left
one), which shows the amount of signal which I had seen before. The
after-fix pictures show very little of that.
Has anyone tried other fixes before? (I'm going to give glutaraldelyde a
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