de-yolking early embryos for microscopy

Megan Roos mroos at neuro.biol.ethz.ch
Tue Jan 7 10:22:47 EST 1997


Hello all.
I am doing some microscopy on wholemount in situ stained embryos of 
8-24H development. I am trying to flatten some out to look at a dorsal 
aspect and having great trouble with interfering yolk grains. Other 
than microdissecting each individual yolk grain away (which is what 
I'm doing), does anyone know of a clever way of getting rid of them 
without destroying my specimen in the process?
Many thanks for any ideas.
Marc.




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