staining buffer

[Shuhong Li]

Dear colleagues:

In prepairing the staining buffer in the in situ hybridization on zf
embryos (100mM NaCl, 100mM Tris-Cl, pH 9.5, 50 mM MgCl2, 0.1% Tween20),
I always get precipitate. Am I wrong in the prepairing steps?

Thanks for any suggestions.

Shuhong Li