staining buffer

Shuhong Li lish at PUBLIC.EAST.CN.NET
Thu Sep 4 04:28:26 EST 1997

Dear colleagues:

In prepairing the staining buffer in the in situ hybridization on zf
embryos (100mM NaCl, 100mM Tris-Cl, pH 9.5, 50 mM MgCl2, 0.1% Tween20),
I always get precipitate. Am I wrong in the prepairing steps?

Thanks for any suggestions.

Shuhong Li

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