POD staining

[Michael Lardelli]

Hi out there,

I'm looking for a little advice about POD staining in zebrafish embryos. We
have been trying to use POD-coupled anti-fluorescein Fab fragments against
fluorescein-labelled cRNA probes with DAB reagents (ready dissolved - all
from Boehringer Mannheim) as a second stain after staining with alkaline
phosphatase-coupled anti-digoxigenin against DIG-labelled cRNA probes.
Background is low as shown by antibody only (no probe) controls. However,
when we develop the colour reaction we see - ZIP - for the POD. Has anyone
had any similar experiences or does anyone have a protocol that they know
works?

I would be very grateful for any suggestions!

Best wishes,

Michael

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Michael Lardelli
Department of Genetics
University of Adelaide
Adelaide
South Australia, 5005
AUSTRALIA

Tel.    (Aust. = 61) (0)8 8303 3212  direct
        (Aust. = 61) (0)8 8303 5633  departmental secretary
Fax.    (Aust. = 61) (0)8 8303 4399
e-mail. mlardelli at genetics.adelaide.edu.au

Visit our website at:
http://www.science.adelaide.edu.au/genetics/lardelli/lardellilabwww
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