monoclonal antibody

Devin Leake dleake at
Thu Jun 4 13:17:09 EST 1998

I am having trouble with immunohistochemistry on whole mount zebrafish embryos.  My 
monoclonal antibody is cross-reacting with the surface of the embryo.  The exact same 
protocol (and secondary antibody)works beautifully with a different monoclonal antibody 
(store bought).  I would appreciate any thoughts on the background staining that I am 
experiencing -- has anyone encountered this before?

dleake at

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