Ubiquitous GFP expression

John Davidson davidson at mbcrr.harvard.edu
Mon Sep 14 17:20:21 EST 1998


My question concerns the maximum intensity of the fluorescence that can
be developed by overexpression of GFP.  Is it toxic at high expression?
Does the adult fish visibly glow when excited at the appropriate
wavelength and does fluorecence diminish as the fish ages?  What
promotors produce the brightest whole body flourescence?

Answers to these questions would be mightily appreciated.

John Davidson

More information about the Zbrafish mailing list