Cloning in Fish--Nucleocytoplasmic Hybrids

[shaoyi yan]

The book publisher asked me to deliver the introduction of the book
"Cloning in Fish" and its Order Form to our community to see if any one
is interested in order it.  If anyone has a trouble to contact with them
who will be welcomed to contact with me and get a 10% discount of book
price. The registered air mail postage is about USD8.00.Thanks for your
help. SY Yan: E-mail:yan.sy at info.iuol.cn.net

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    Cloning in Fish--Nucleocytoplasmic Hybrids

Written by: Shaoyi Yan DSc. , Senior Research Professor, Institute of
Developmental Biology, the Chinese Academy of Sciences, Beijing, China
with editorial suggestions from Marie A. Di Berardino, Ph.D.,
J.D.,Professor Emerita of Biochemistry, Medical College of
Pennsylvania-Hahnemann School of Medicine, Allegheny University of the
Health Sciences, Philadelphia, Pennsylvania, USA

Dr. T.S. Okada, Director, The JT Biohistory Hall, Prof. Emerita., Kyoto
University, Japan; Ex-President of the International Society of
Developmental Biologists; Ex-Vice President of the International Union
of Biological Sciences, wrote an Introductory Remarks for this book..
The publication of this book was sponsored by the International Union of
Biological Sciences, Paris, France and other Foundations in China.
June 1998  150 pages  HB
ISBN: 962-8182-10-2
Price: USD 28.00

Brief introduction of this book:
Animal cloning involves non-sexual methods of reproduction. one method
is nuclear transplantation or cell fusion in which the nucleus of a
developmentally totipotent cell is transferred into an enucleated egg
(egg’s nucleus is removed or inactivated) of the same or different
species. In successful cases, the eggs develop into animals whose
nuclear genotypes is identical to that of the respective transplanted
nucleus. This type of animal cloning was initiated in the 1950’s in
amphibians, and later extended to fish (1960’s), insects (1970’s) and
mammals (1980’s). In all those groups normal animals resulted from
intraspecific clones. However, interspecific hybrid cloning in
amphibians and mammals was successful in only a few closed related
amphibians. But in fish, fertile hybrid clones from distantly related
species were obtained and, in some cases, displayed modified and
improved phenotypic characteristics. The studies of interspecific clones
also provide important information on understanding interactions between
the nucleus (genome) and cytoplasm, and the consequences of the
interactions during ontogenesis. The cloning method itself could be used
to replicate transgenic animals, thereby augmenting the quantity of
useful models for fundamental biological research, and to produce new
breeds of fish clones important for aquaculture.

This book reviews the research on fish cloning from the 1960’s to the
present, and compares the results to that in amphibians and mammals. It
includes four chapters of text; thirty black and white and twenty one
color pictures. In addition, it contains a detailed bibliography and
manual of experimental techniques. It will be a useful reference for
investigators, teachers and students in universities, who are working or
interested in basic research in biology and biotechnology.

Contents:
I.INTRODUCTION.
II.MATERIALS AND METHODS.
    1. Fish stocks
    2. Spawning seasons of fish
    3. Method for artificial spawning
    4. Some special features and behavior of fish eggs cultured in vitro

    5. Nuclear transplantation procedures
    6. Fertilization and early development of fish.
    7. Preparation of mouse blastomeres from the 16?cell stage
     8. Chromosome examination of fish embryos
    9. Technique for scanning electromicroscopy
    10.Technique for immuno-electrophoresis
   11.Technique for starch electrophoresis
    12.Technique for two-dimensions polyacrylamide gel electrophoresis
    13.Technique for disc polyacrylamide gel electrophoresis
    14.Technique for DNA reassociation kinetics
    15.Technique for isolation and identification of mitochondrial DNA
    16. Technique for DNA dot hybridization
III. EXPERIMENTAL RESULTS.
     1.Inter-variety combination
     2.Inter-genus combination
    3.Inter-subfamily combination
     4.Inter-family combination
     5.Inter-order combination
     6.Inter-class combination
     7.Results of nuclear transplantation in fish reported by other
authors in China
     8.Results of nuclear transplantation in fish reported by authors in
formal USSR
IV. DISCUSSION
 V. ACKNOWLEDGMENT.
IV. REFERENCES
        IIV.INDEX

 Name list of fish used in nuclear transplantation:

  1.  Goldfish (Carassius auratus, domestic type)
  2.  Crucian carp (Carrassius auratus, wild type)
  3.  Common carp ( Cyprinus carpio)
  4.  Grass carp ( Ctenopharyngodon idellus)
  5.  Silver carp (Hypophthalmichihys molivrix)
  6.  Blunt-snout bream (Megalobrama amblycephals)
  7.  Chinese bittering (Rhoedus sinensis)
  8.  Large scale loach (Paramisgurnus dabryaus)

   9. Loach Misgurnus anguillicaudatus)
  10. Zebrafish (Brachydanio rerio)
  11. Tilapia (Orechromis nilotica)
  12. Mud carp (Cirrhinus molitorella)


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