Requesting help keeping young fish alive in small
bxchapman at ucdavis.edu
Mon Aug 9 14:11:56 EST 1999
Dear Zebrafish community--
I am currently involved with a NASA project trying to send
embryos into space (on NASAs space shuttle) in order to study
of microgravity on the development of the vestibular system.
The problem we are having is that we need to keep embryos
alive for 19
days in a very small volume of liquid (35 mls). Due to the
the hardware, there is no way to have access to the aquaria to
water, etc. during flight. The only possibility to add anything to the
aquaria during flight is through an automatic mechanism (designed
injecting fixative, but which we will not be using for that purpose)
can inject 1 ml of liquid once during the flight.
What we do now: Currently the fish are allowed to develop
28.5 degrees C until 10 hours post fertilization. At that point we
the chorions enzymatically using pronase, and transfer the
Hanks solution containing PTU (to prevent melanin formation).
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