In situ hybridization

Angela Manning a.manning at ualberta.ca
Mon Feb 22 18:49:17 EST 1999


Hi, I would like to know if anyone out there has used a 3'RACE product as a
probe for in situ hybridization.  The reason I'm asking is I'm trying to
decide if the poly A tail will interfere with the specificity of the probe.
 The complete length of the probe will be 600 bp and the poly A tail is
less than 20 nts so I don't think there should be a problem, but...better
safe than sorry!  Thanks, Angela

-- 
Angela Manning       a.manning at ualberta.ca
Dept of Biological Sciences
University of Alberta
Edmonton, AB Canada
T6G 2H3




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