in situ without melanocytes

[Herwig Baier]

Hi Petur,
you can suppress melanization in zebrafish embryos for up to a week by bath
application of phenyl thiocarbamide (or phenyl thiourea/PTU; 200uM)
starting on the morning of day 2.

Good luck,
-Herwig

>    I am thinking about doing an in situ on zebrafish emryos but am
>afraid that the melanocytes might interfere with my staining, I have
>heard that there is a method to somehow fix this. Anyone heard of this?
>
>petur petersen
>
>email: petur.petersen at yale.edu


Herwig Baier

University of California, San Francisco
Department of Physiology
Programs in Neuroscience, Genetics, and Human Genetics
513 Parnassus Avenue, Room S-762
San Francisco, CA 94143-0444

Email: hbaier at itsa.ucsf.edu
Phone: +1(415)502-4301
Fax: +1(415)476-4929