IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

reporter genes and cell lines

Colin Dolphin colin.dolphin at kcl.ac.uk
Fri Jun 25 10:08:21 EST 1999

I am writing a grant with the ultimate aim of defining the molecular
mechanism underlying the osmoregulatory control of certain flounder
(yes, I know this is a zebrafish news group but I thought someone might
be able to help) genes we aim to isolate. Although I have no problem
with the basic methods to be used I don't have direct, hands-on
experience of reporter gene assays and certainly wouldn't know about any
suitable responsive fish cell lines. Can anyone help with ideas on
reporter genes and a suitable cell line which might be responsive to
changes in external salinity and could be used for deletion mutant
analysis ?

Colin Dolphin
Department of Pharmacy
King's College London
Manresa Road
London SW3 6LX

Tel (44) 171 333 4806
Fax (44) 171 351 5307
email colin.dolphin at kcl.ac.uk

More information about the Zbrafish mailing list

Send comments to us at biosci-help [At] net.bio.net