In situ hybridization in zebrafish

Angela Manning a.manning at ualberta.ca
Wed Nov 10 17:46:07 EST 1999


Hi, I normally use NBT & BCIP (alkaline phosphatase) as my 
detection in zebrafish wholemount in situ hybridizations.  I've heard 
that TNBT and BCIP can give a much darker precipitate and so I've 
decided to use it instead of the NBT.  However, I want to make 
sure that it should be dissolved in 70% dimethylformamide as NBT 
is.  Can anyone confirm this for me?  Also if the solution must be 
warmed up etc.?  Thanks in advance,
Angela

-- 
Angela Manning       a.manning at ualberta.ca
Dept of Biological Sciences
University of Alberta
Edmonton, AB Canada
T6G 2H3






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