raising dechorionated embryos

pam kainz pkainz at fas.harvard.edu
Fri Nov 19 11:34:31 EST 1999


Has anyone had luck in raising zebrafish embryos that have been
dechorionated?  I've been getting about 60% to live past the critical 
day 8-10 period but hope to find a way to increase this percentage.

The protocol I am currently using is as follows:

    - dechorionate embryos at 10 hpf
    - raise them in embryo medium
    - from day 3 to day 5, gradually replace the embryo medium with 
fish water
    - begin feeding on day 5

Pam Kainz
Dept. of Molecular and Cellular Biology
Harvard University
16 Divinity Ave.
Cambridge, MA 02138
(617) 495-2599





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