raising dechorionated embryos

[pam kainz]

Has anyone had luck in raising zebrafish embryos that have been
dechorionated?  I've been getting about 60% to live past the critical 
day 8-10 period but hope to find a way to increase this percentage.

The protocol I am currently using is as follows:

    - dechorionate embryos at 10 hpf
    - raise them in embryo medium
    - from day 3 to day 5, gradually replace the embryo medium with 
fish water
    - begin feeding on day 5

Pam Kainz
Dept. of Molecular and Cellular Biology
Harvard University
16 Divinity Ave.
Cambridge, MA 02138
(617) 495-2599