raising dechorionated embryos
pkainz at fas.harvard.edu
Fri Nov 19 11:34:31 EST 1999
Has anyone had luck in raising zebrafish embryos that have been
dechorionated? I've been getting about 60% to live past the critical
day 8-10 period but hope to find a way to increase this percentage.
The protocol I am currently using is as follows:
- dechorionate embryos at 10 hpf
- raise them in embryo medium
- from day 3 to day 5, gradually replace the embryo medium with
- begin feeding on day 5
Dept. of Molecular and Cellular Biology
16 Divinity Ave.
Cambridge, MA 02138
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