baiwei at jhs.com.sg
Mon Nov 29 15:08:07 EST 1999
I am a part-time student of Dept. of Biological Sciences, National
University of Sinagpore. I use zebrafish embryos in my
microinjection experiment. I meet a big problem of my fish embryos.
My zebrafish embryos are breeded by 5-months old fish and seem
OK for growing. But when I use them for injecting, I find they are
very easy to die. If I observe them carefully under Olympus
dissecting microscope, I find the one-to 16 -cell stage eggs are all
very ugly, they are something not smooth on the YOLK surface
and the cell is not clear and in good shape, but they are still alive
and can grow to a "fish".
I do not know how to do, because I change a lot of batches of 5-
months old fish, get the same problem. And it should not be
caused by my injecting DNA and even be injected with water, they
will die. I had a good batches of fish before half a year, I knew what
is a good eggs, but I use the same method to treat them. I feed
them with frozen red worm. Only change is that last time, I change
water frequently about 2days one time, now I clean the tanks about
1 week on time to my new batch fish . Is it important?
Can you give me some suggestion?
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