Nickel

Leo van der Ven l.vd.ven at hccnet.nl
Mon Feb 28 17:08:21 EST 2000


I used 35 mg DAB with 250 mg NiCl2.6H2O, 875 mg NaCl, 153 mg
Na2HPO4.2H2O, 19.5 mg NaH2PO4.H2O in 100 ml AD, pH 7.4, 
and 0.1 ml 30% H2O2 (e.g. van der Ven et al. Histochem Cell Biol 
1997, 107:193-203). Counterstain with nuclear fast red (0.1% in a 
5% Al2(SO4)2.18H2O solution)

Veronica wrote:
> 
> I would like to alter the DAB reaction by adding Nickel... But which is
> the optimal concentration to use?
> 
>  Thanks in advance,
> 
>  Veronica Garcia
---






More information about the Zbrafish mailing list