Detecting FLAG- and HA-tagged proteins by whole-mount
lwillis at itsa.ucsf.edu
Thu May 18 18:19:16 EST 2000
I'm doing some DNA injections into Zebrafish embryos and I need
to verify the tissue-specific expression of my proteins. I now that
myc tags are most oftgen used in Zebrafish, however my genes are
already FLAG and/or HA tagged.
Has anyone had FLAG (M2 antibody) or HA (12Ca5 antibody)
whole-mounts work or NOT work?
Thank you , David Willison
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