Detection level for whole mount in situ

Michael Lardelli michael.lardelli at
Tue Apr 10 12:29:41 EST 2001

Hi out there!

Can anyone direct me to a reference that gives some statement as 
to the lower detection threshold for whole mount in situ transcript 
hybridization on zebrafish (or any other) embryos? i.e. What is the 
number of transcripts per cell below which whole mount in situ will 
not give a signal. Even a crude estimate would be appreciated. 
Thanks for your help!  


Michael Lardelli
Department of Molecular Biosciences
Discipline of Genetics and
   Special Research Centre for the
   Molecular Genetics of Development
Adelaide University

Tel.    (Aust. = 61) (0)8 8303 3212  direct
        (Aust. = 61) (0)8 8303 5633  departmental secretary
Fax.    (Aust. = 61) (0)8 8303 4399
e-mail. michael.lardelli at

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