Freezing Sperm

[matt frieda]

Hello,

I am looking for a sperm freezing/thawing protocol that produces a 
high fertilization rate.  I am thinking of freezing crushed testes in 
some sort of buffer. Has anyone done this? Does anyone know of a 
protocol or paper that desribes a method that produces good 
fertilization? I have tried the protocol listed in the Zebrafish Book 
but it has not produced a high fertilization rate in my hands.  

Thank you.

Matt Frieda
Henion/Beattie Zebrafish Lab
Ohio State University
mfrieda at genetics.wustl.edu