problems with zebrafish embryos
Dallas Weaver, Ph.D.
deweaver at gte.net
Mon Dec 10 11:05:01 EST 2001
The most common problem is overfeeding with poor water quality a
close second (often caused by overfeeding). Check the ammonia
levels. Live feed is preferable.
in article 9u8ato$hsa$1 at mercury.hgmp.mrc.ac.uk, triona hart at
triona_ at hotmail.com wrote on 11/30/01 8:08 AM:
> I am an undergraduate at Bard College. We recently recieved a
> shipment of 100 ABC wild type zebrafish embryos from the
> Zebrafish Resource Center. We were unable to keep them alive in our
> lab despite efforts to follow directions in the ZF book.
> Embryos were recieved on Friday Nov 16 and all of them had died 6
> days later on Thursday Nov 22. They were kept at 27 degrees C. One
> third of their water was changed daily. They were fed liquifry. Only
> aged tap water (ph 7.6) was used to house them.
> Does anyone know any common mistakes made when people first
> try to keep embryos? If you see any glaring problems with what we
> did it would be a great help for you to point them out. Thanks so
> Triona Hart
> Bard College
> P.O. Box 5000
> Annandale-on-Hudson, NY 12504
> elephone: (845) 752-4198
> triona_ at hotmail.com
------- End of forwarded message -------
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