problems with zebrafish embryos

Dallas Weaver, Ph.D. deweaver at gte.net
Mon Dec 10 11:05:01 EST 2001


The most common problem is overfeeding with poor water quality a 
close second (often caused by overfeeding).  Check the ammonia 
levels.  Live feed is preferable.  

Dallas


in article 9u8ato$hsa$1 at mercury.hgmp.mrc.ac.uk, triona hart at
triona_ at hotmail.com wrote on 11/30/01 8:08 AM:

> I am an undergraduate at Bard College.  We recently recieved a
> shipment of 100 ABC wild type zebrafish embryos from the
> Zebrafish Resource Center. We were unable to keep them alive in our
> lab despite efforts to follow directions in the ZF book.
> 
> Embryos were recieved on Friday Nov 16 and all of them had died 6
> days later on Thursday Nov 22. They were kept at 27 degrees C. One
> third of their water was changed daily. They were fed liquifry. Only
> aged tap water (ph 7.6) was used to house them.
> 
> Does anyone know any common mistakes made when people first
> try to keep embryos?  If you see any glaring problems with what we
> did it would be a great help for you to point them out. Thanks so
> much!  
> 
> Sincerely,  
> 
> Triona Hart
> Bard College
> P.O. Box 5000
> Annandale-on-Hudson, NY 12504
> elephone: (845) 752-4198
> triona_ at hotmail.com
> 
> 

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