In situ probes

Angela Manning a.manning at ualberta.ca
Fri Feb 2 15:38:15 EST 2001


Can anyone offer advice on making DIG labelled RNA probes for in situ
hybridization in zebrafish (wholemount) embryos?

Specifically the optimal size of the probe, the optimal content (coding vs.
UTR), how to check the probe before use (eg. quantitating and checking to
ensure the probe is intact), the optimal concentration of probe to use in
the experiment and also what controls are really the most informative (eg
sense or leaving the probe out completely).  Thanks very much!

-- 
Angela Manning       a.manning at ualberta.ca
Dept of Biological Sciences
University of Alberta
Edmonton, AB Canada
T6G 2H3








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