EGFP flourescence after fixation

Greg Conway gconway at
Fri Jul 13 16:47:30 EST 2001

Has anyone gotten good EGFP fluorescence after fixation? And 
what fixative and conditions do people use to preserve the 
fluorescence with minimal background?

Also,  which GFP antibody (commercially available?) is best for 
visualizing GFP expression post-fixation with minimal background?


Greg Conway, Ph.D.
Principal Investigator
Mail Stop 239-11
NASA Ames Research Center
Moffett Field, CA 94035
Phone: (650) 604-3595
Fax: (650) 604-3954

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