immunos and pigmentation
Declan W Ali
declan.ali at ualberta.ca
Thu Jun 14 16:45:38 EST 2001
My name is Declan Ali and research in my lab focuses on the
physiology of synaptic development. We use zebrafish as our
organism of study and have recently taken up performing
immunohistochemistry on wholemounts of embryos and larvae. We
are having some trouble with background and transparency in older
animals (3-5 days), particularly with the presence of pigmentation.
We rear larvae in 0.03% PTU which retards pigmentation but I am
not happy with the strength of the specific staining in PTU, and
was wondering if you knew any other method of clearing pigments.
Since our specific staing is a bit weak it doesn't help that the
background and pigmentation is high in older animals. We use
FITC or Cy3 coupled to our secondary and clear the wholemounts
Can someone help us?
Declan W Ali, PhD
Assistant Professor of Zoology
University of Alberta
Department of Biological Sciences
CW-405 Biological Sciences Bldg.
Canada, T6G 2E9
Tel: (780) 492-6094
Fax: (780) 492-9234
Email: declan.ali at ualberta.ca
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