question about fixation
bobice_guan at yahoo.com
Fri May 4 12:57:29 EST 2001
I'm a student from Fudan University, and I am doing some in situ
Hybridization in zebrafish embryo. The following is my question
Since PFA's role is to kill RNAse, so fix for longer time (such as
Overnight) at Room Temperature is better. My in situ has proved it.
But why your protocol suggest fix O/N at 4 =B0C?
I'm looking forward your reply soon.
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