Embryo medium

"Peter Cattin PhD p.cattin at auckland.ac.nz
Fri Nov 23 17:07:59 EST 2001

Hi Yamila,

The E3 medium works well.  It is made as follows:
14.61g NaCl/l
0.63 g KCl/l
2.43 g CaCl2/l
4.07 g MgSO4/l

Put in a petri dish with 50 eggs in an incubator at 28 degrees C.  
Add methylene blue at 2-4mg/l to keep the fungus and bacteria 
under control.  This works very well but should still be replaced by 
removing the old with a pipette and replacing with fresh every 
couple of days. Start feeding day 3 or 4.  

Good luck.

Peter Cattin
Peter Cattin PhD
Division of Molecular Medicine
Faculty of Medical and Health Sciences
University of Auckland
Park Road, Grafton, Auckland.
Private bag 92019, Auckland,
New Zealand.

Tel: +64-9-373-7599 xtn. 6373
Fax: +64-9-373-7492

Yamila Carpio Gonzales wrote:

> Hi,
> Please suggest me an embryo medium that works well.
> Thanks
> Yamila
> ---

More information about the Zbrafish mailing list