"Peter Cattin PhD
p.cattin at auckland.ac.nz
Fri Nov 23 17:07:59 EST 2001
The E3 medium works well. It is made as follows:
0.63 g KCl/l
2.43 g CaCl2/l
4.07 g MgSO4/l
Put in a petri dish with 50 eggs in an incubator at 28 degrees C.
Add methylene blue at 2-4mg/l to keep the fungus and bacteria
under control. This works very well but should still be replaced by
removing the old with a pipette and replacing with fresh every
couple of days. Start feeding day 3 or 4.
Peter Cattin PhD
Division of Molecular Medicine
Faculty of Medical and Health Sciences
University of Auckland
Park Road, Grafton, Auckland.
Private bag 92019, Auckland,
Tel: +64-9-373-7599 xtn. 6373
Yamila Carpio Gonzales wrote:
> Please suggest me an embryo medium that works well.
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