Embryo medium

["Peter Cattin PhD]

Hi Yamila,

The E3 medium works well.  It is made as follows:
14.61g NaCl/l
0.63 g KCl/l
2.43 g CaCl2/l
4.07 g MgSO4/l

Put in a petri dish with 50 eggs in an incubator at 28 degrees C.  
Add methylene blue at 2-4mg/l to keep the fungus and bacteria 
under control.  This works very well but should still be replaced by 
removing the old with a pipette and replacing with fresh every 
couple of days. Start feeding day 3 or 4.  

Good luck.

Peter Cattin
--
Peter Cattin PhD
Division of Molecular Medicine
Faculty of Medical and Health Sciences
University of Auckland
Park Road, Grafton, Auckland.
Private bag 92019, Auckland,
New Zealand.

Tel: +64-9-373-7599 xtn. 6373
Fax: +64-9-373-7492

Yamila Carpio Gonzales wrote:

> Hi,
> Please suggest me an embryo medium that works well.
> Thanks
> Yamila
>
> ---