MICROINJECTION

[tcbecker]

Hi Johana,

what you can try is to play with the holding pressure on your
injector. I find that with a slightly negative holding pressure, the
fluid is not drawn up the capillary and does not leak either.

Good luck

Catherina

Johana Kuncova wrote:

> Dear all!
>
> I would like to turn to those who are using capillary pressure
> microinjection technique. I am currently trying to calibrate the
> system and also to do some injections into adherent cells.
>
> I have noticed that the capillary, once immersed into the medium,
> where the cells are, starts leaking. Is there any way to avoid it?
>
> Thank you in advance for your answers,
>
> Johana
>
> P.S.: I would be also interested in comments on use of the
> microinjectors, so if you happen to have one, please, pop out of the
> crowd. THX
>
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