hank's solutions

Stephen Devoto sdevoto at wesleyan.edu
Mon Oct 29 17:02:52 EST 2001

Depending on the size of your system, you will find Hank's to be 
prohibitively expensive in terms of labor and materials. We use 
reverse osmosis (RO) water (commonly called "single-distilled" or 
"dH2O" in labs here).  We add Instant Ocean to a conductivity of 
600 microSiemens. This corresponds to about 0.4 g per liter.  We 
also add a lot of Arm and Hammer Baking Soda (bicarbonate) to 
buffer the pH. The advantage of Instant Ocean is that it (in principle) 
has lots of the trace minerals which fishes need for optimal vitality 
(minerals present in tap water but removed by RO).  We make up a 
super-concentrated Instant Ocean solution and add that to RO 
water to make our "fish water" Stephen.  

Christina Joselevitch wrote:

> The tap water of our institute is contaminated with copper, so we
> thought about adding salts to distilled water in order to keep our
> (adult) zebrafish happy. The problem is that we don't know which
> salts/ how much salt. I would like to know whether I could use
> Hank's solution in our aquaria?
> Thanks,
> ><((((=BA>`=B7.=B8=B8.=B7=B4=AF`=B7.=B8.=B7=B4=AF`=B7...=B8><((((=BA
> >>=B8.
> Christina Joselevitch
> ><((((=BA>`=B7.=B8=B8.=B7=B4=AF`=B7.=B8.=B7=B4=AF`=B7...=B8><((((=BA
> >>=B8.
> The Netherlands Ophthalmic Research Institute
> Meibergdreef 47
> 1105 BA Amsterdam
> The Netherlands
> phone: + 31 20 566 5104 or + 31 20 566 5119
> fax:   + 31 20 566 6121
> email: c.joselevitch at ioi.knaw.nl
> http://pc4.ioi.knaw.nl/~kamermans/
> =B7=B4=AF`=B7.=B8. , . ..=B7=B4=AF`=B7..
> ><((((=BA>`=B7.=B8=B8.=B7=B4=AF`=B7=
> .=B8.=B7=B4=AF`=B7...=B8><((((=BA>
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