hank's solutions

[Dr Peter Cattin]

Christina,

You have had a very detailed response already and I am sure it 
works but perhaps a simpler option may be to use distilled or 
reverse osmosis (RO) produced water and add a mixture of 1 part 
NaHCO3, 2 parts CaCL2.2H2O and 1 part Instant Ocean to give a 
final conductivity of 400 - 800 mS.  This solution will be fairly stable 
from a buffering perspective. Unfortunately, you have to start with 
"pure" water.  RO is an option although it has a low % recovery but 
is otherwise cheap to run (just a pump and membrane 
replacement) while distillation has a high recovery but also has 
fairly high operating cost (electricity).  

Cheers,

Peter

--
Peter Cattin PhD
Division of Molecular Medicine
Faculty of Medical and Health Sciences
University of Auckland
Park Road, Grafton, Auckland.
Private bag 92019, Auckland,
New Zealand.

Tel: +64-9-373-7599 xtn. 6373
Fax: +64-9-373-7492

Christina Joselevitch wrote:

> The tap water of our institute is contaminated with copper, so we
> thought about adding salts to distilled water in order to keep our
> (adult) zebrafish happy. The problem is that we don't know which
> salts/ how much salt. I would like to know whether I could use
> Hank's solution in our aquaria?
>
> Thanks,
> ><((((=BA>`=B7.=B8=B8.=B7=B4=AF`=B7.=B8.=B7=B4=AF`=B7...=B8><((((=BA
> >>=B8.
>
> Christina Joselevitch
>
> ><((((=BA>`=B7.=B8=B8.=B7=B4=AF`=B7.=B8.=B7=B4=AF`=B7...=B8><((((=BA
> >>=B8.
> The Netherlands Ophthalmic Research Institute
> Meibergdreef 47
> 1105 BA Amsterdam
> The Netherlands
> phone: + 31 20 566 5104 or + 31 20 566 5119
> fax:   + 31 20 566 6121
> email: c.joselevitch at ioi.knaw.nl
> http://pc4.ioi.knaw.nl/~kamermans/
> =B7=B4=AF`=B7.=B8. , . ..=B7=B4=AF`=B7..
> ><((((=BA>`=B7.=B8=B8.=B7=B4=AF`=B7=
> .=B8.=B7=B4=AF`=B7...=B8><((((=BA>



---
------- End of forwarded message -------