Announcement for the NIH BAC Sequencing Program

Graham, Bettie (NHGRI) grahambj at exchange.nih.gov
Thu Sep 6 09:59:44 EST 2001


OPPORTUNITY FOR OBTAINING DNA SEQUENCE OF REGIONS 
OF HIGH BIOMEDICAL INTEREST FROM MODEL ORGANISM 
GENOMES  

Next Submission Date: October 1, 2001
ACCESS TO FACILITIES 

The National Institutes of Health has expanded the list of 
organisms eligible for sequencing under the current NIH Mouse 
BAC Sequencing Program to include all animals, fungi, and 
eukaryotic protists (http://grants.nih.gov/grants/guide/notice-
files/NOT-HG-01-002.html).  This program change is intended to 
address the interest of the larger biomedical research community 
in obtaining sequence information about specific regions of 
genomic DNA of biomedical or biological significance.   

Several of the sequencing centers in the National Human Genome 
Research Institute's Genome Sequencing Network will dedicate a 
fraction of their sequencing capacity to this initiative. Investigators 
may submit requests to have one or more BAC clones sequenced 
from one or more eligible organisms (plants and prokaryotes are 
excluded). Requests may be for 4-fold coverage, 6-10-fold 
coverage, or finished sequence.   Any BAC sequenced from the 
mouse RPCI-23 library or the rat BAC library designated for 
genomic sequencing will be finished so that the sequence will be 
maximally useful to the centers generating the sequence of the 
entire genome. Requestors whose projects have been approved 
must also provide the BAC clone(s) to the participating sequencing 
centers.  

An Investigator interested in obtaining the sequence of a specific 
region of genomic DNA that has been cloned in a BAC may submit 
a short, Web-based request describing the region, its importance, 
and its readiness to be sequenced.   A panel of peer reviewers will 
consider the requests and advise the NHGRI on the priority of the 
regions requested.  Those judged to be sufficiently important to 
warrant priority sequencing will be listed for the centers engaged in 
mouse genomic sequencing to choose and sequence, up to the 
maximum capacity available for this activity.   

There will be no cost to investigators seeking this sequencing 
service; the sequencing will be done by centers that have already 
been funded through the Network.  However, as with all sequence 
data generated by the Human Genome Project, unfinished data will 
be submitted to GenBank within 24 hours of generation of 2kb 
assemblies, and finished data as soon as completed.   No 
sequence data will be made available to the requestor prior to 
public release.   All publications using this data must acknowledge 
the publicly funded sequencing effort.  If BAC clones that are 
approved for sequencing are not available commercially, the 
requestor must agree to make the clone(s) available for distribution 
in an expeditious manner upon publication using any or all of the 
data generated by the public effort.  

For a more complete description of the program and to access the
request form, please visit our website: http://mouse.info.nih.gov
<http://mouse.info.nih.gov> .


To discuss programmatic issues contact:
Bettie J. Graham, Ph.D. 
National Institutes of Health; Bethesda, MD 20892-2033
Email: bettie_graham at nih.gov; <mailto:bettie_graham at nih.gov;>  
TEL:
(301) 496-7531

To discuss review issues contact:
Jerry Roberts, Ph.D.  
National Institutes of Health; Bethesda, MD 20892-2032 
E-mail:  jerry_roberts at nhgri.nih.gov; TEL: (301) 402-0838





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