Zebrafish colony start-up problem
Beth Ann Sipple
bsipple at stvincent.edu
Mon Aug 12 09:47:32 EST 2002
Hi fellow zfish researchers,
My name is Beth Sipple and I am currently starting up a zfish
colony at Saint Vincent College for my student Betsy who is
investigating various aspects of early heart development.
However, we hit a snag today with the colony and I was hoping that
I might be able to get some hints from you all on what to do.
The tanks were thoroughly cleaned & filled according to the regular
zfish guidelines. The 10gal tanks ran with the correct size filter for
3 weeks prior to introducing the fish. All 40 fish were placed into
one of the tanks and they were to be subdivided in one week. pH
and temp have been stable. My student has been in charge of
tank maintenance and she updates me daily. However, after 5
days the water became cloudy, whitish cloudy not algae cloudy but
the fish seemed fine. I planned on contacting PetSmart on
monday to see what I should do to help clear the water but there
did not appear to be an urgency since the fish appeared active &
healthy. Then a call from Betsy today (Sunday) informed me of a
few deaths. I had her move the fish to the back up tank (on a
separate water supply) and to put a water sample and the dead fish
in the frig. The fish and water are going to Pet Smart tomorrow
afternoon (we are not using any standard wild type strains like *AB
for her work). They have a 14 day warrantee (that sounds odd
doesn't it). Anyway, I am not sure what happened but I am
thinking that 1) overfeeding led to a build up of by-products or 2) the
filter was not able to handle clearing the wastes from 40 fish in the
one 10gal tank.
It has been over 4 years since I have handled fish and I don't even
have a tank at home. Any hints or suggestions on what might be
wrong and how to fix it would be extremely helpful!!!
Thank you for your help in advance!
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