jpilger at agnesscott.edu
Tue Aug 27 10:36:12 EST 2002
Dear Z'fisher persons,
I have two questions.
First, I want to check on one of the recipes in the Zebrafish Book
that I believe may be incorrect. It is a component of the Embryo
Medium, Hank's Stock #2 Solution. In the book it states the
following recipe: Hank's Stock #2 0.358 g Na2HPO4 Anhydrous
0.60 g K2H2PO4 in 100 ml ddH2O
I don't think that K2H2PO4 is a valid compound and wonder if this
is meant to be either KH2PO4 or K2HPO4? (Personally, I think it
might be the former of the two.)
Second, the Western Blot protocol described in the Zebrafish Book
(section 9.4) indicates using a PBDF transfer membrane. I haven't
been able to locate this type and wonder if this might be a
typographical error and should be PVBF?
I hope someone can answer these questions?
Thank you in advance.
John F. Pilger, Ph.D.
Professor of Biology
Agnes Scott College
141 E. College Ave.
Decatur, GA 30030-3797
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