Overexpression in zebrafish embryos

tcbecker tcbecker at zmnh.uni-hamburg.de
Wed Feb 6 17:28:35 EST 2002


Dear All,

For a while now we have been trying to overexpress various 
constructs by RNA injections into zebrafish eggs. We want the 
protein to be around for about 24 hours. While we were successful 
with relatively short constructs (appr. 500bp) as judged by the 
detection of the myc tag, longer constructs (1 kb and up) were 
never detectable after a day.  

Are you aware of any tricks to stabilize the RNA and get it reliably
expressed? Any info is appreciated.

Thank you!

Catherina Becker





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