chi-bin.chien at hsc.utah.edu
Mon Jul 22 10:26:23 EST 2002
I don't have any direct experience with fish cell lines, but
corresponded several months ago with David Traver in the Zon
laboratory. I am assuming that he won't mind if I pass on his reply
(below). It sounds like the ZF4 line is worth trying.
On 23 Jan 2002 David Traver wrote:
>I have done a little bit with zf fibroblast-like lines, namely ZF4
>which was derived from embryos by Wolfgang Driever and AB9 which was
>derived from Barry Paw in our lab from adult fins. They both grow at
>28.5, ZF4 much faster than AB9 which is more like a primary line,
>i.e. pain in the ass to grow.
>I believe you can get both from ATCC.
>David Traver, PhD.
At 6:18 PM +0100 7/17/02, Jill Murtha wrote:
>Hello! Does anyone have any experience with using zebrafish cell
>lines? I've been trying to get the AB.9 cells (fibroblast line from
>ATCC) going and have been having a terrible time. They never reach
>confluency and start dying off a few days after I start them up
>immediately upon receipt. ATCC has sent me a couple of replacements,
>but the problem is still the same. I'm following their
>recommendations exactly, and I've spoken to their tech support a
>couple of times, but I can't figure out what is wrong. I'd
>appreciate it if anyone who has any experience with this cell line or
>any other zfish cell lines would be willing to share their
>experiences and advice with me.
>Also, does anyone have any zebrafish cells of any kind growing
>that they would be willing to send me? Either a frozen vial or a
>growing flask would be fine. Of course, we would cover any expenses
>necessary, including shipping. I would be very grateful.
>Jill M. Murtha, DVM
>Post-Doctoral Research Fellow
>Unit for Laboratory Animal Medicine
>University of Michigan
>018 Animal Research Facility
>Ann Arbor, MI 48109-0614
< Dept. Neurobiology and Anatomy | office: 1-801-585-1701 > <
Univ. Utah Medical Center | lab: 1-801-585-1702 > < 20
North 1900 East, 401 MREB | fax: 1-801-581-4233 > < Salt
Lake City, UT 84132 | chi-bin.chien at hsc.utah.edu > <
More information about the Zbrafish