Zfish sectioning

[Brian Perkins]

It depends on what you want to do with them.  Fixation for 
immunohistochemistry in frozen cryosections works best with 4% 
paraformaldehyde at 4 degrees C.  This also works well for plastic 
(alradite resin) sections to be stained.  Alternatively, fish processed 
for plastic sections or electron microscopy sections will often do 
better when fixed in 2.5% glutaraldehyde for one hour at room temp 
(overnight at 4 C) followed by a second fixation with 1% osmium 
tetroxide.  This provides a better contrast of the photoreceptor outer 
segments with the cell bodies and plexiform layers.  
Paraformaldehyde fixation does not allow very good staining of the 
outer segments but does allow better staining of the cell bodies.  

--
*****************************************************
Brian Perkins, Ph.D.
Department of Molecular and Cellular Biology
Harvard University
16 Divinity Ave.
Cambridge, MA  02138
PH:  617-495-2599
Fax:  617-496-3321
bperkins at fas.harvard.edu
*****************************************************
"hsayen" <hsayen at blue.weeg.uiowa.edu> wrote in message
news:ahpk3s$cun$1 at mercury.hgmp.mrc.ac.uk...
>
> My name is David Yen and I am a graduate student @ University of
> Iowa. My thesis project is involved in zebrafish research and
> recently we are going to look at the eye section of the zfish.  I
> just wonder what's the best fix to use to preserve the integrity of
> the eye structure.  And how long should I fix them?  Thanks much and
> have a great day!
>
>
>


---
------- End of forwarded message -------