protein isolation from adult zebrafish tissues

[Jill]

Hello!  I've been trying to isolate protein from adult zebrafish
tissues (brain, liver, and muscle), but I've had some trouble getting
a clear lysate and I'm not too confident in the quality of the samples
I'm getting.  I've been using a protocol from Santa Cruz, but its
assuming that the quantities of tissues being used are much larger
than what I have, so I've had to weigh my tiny samples and scale the
volumes of buffer and enzymes down accordingly.  And I'm not sure how
accurate that is.

Does anyone have a good protocol for extracting total proteins from
adult zebrafish tissue, or from very small quantities of tissues in
general?

Thanks in advance,
Jill

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