Job Posting

[Pollier, Robin]

TWO RESEARCH ASSOCIATE POSITIONS

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	                 RESEARCH ASSOCIATE #1

The Division of Gene Function & Expression at the University of 
Massachusetts Medical School is recruiting for a Research 
Associate who will manage and supervise the care, maintenance 
and husbandry of mutant and transgenic zebrafish lines.

MAJOR RESPONSIBILITIES:  

Responsible for independently managing a zebrafish facility. 
Responsible for the maintenance of mutant and transgenic 
zebrafish lines. Assist  PI in the establishment, maintenance and 
backup of fish facility databases. Isolate and purify DNA from 
zebrafish embryos and adult finclips. Perform polymerase chain 
reaction (PCR) to identify mutant or transgenic carrier zebrafish 
Identify mutant or transgenic carriers by crossing and phenotypic 
scoring of resulting embryos. Perform microinjection of DNA 
constructs into zebrafish embryos to establish stable transgenic 
lines. Perform techniques for cryopreservation of mutant or 
transgenic zebrafish lines. Perform in vitro fertilization using adult 
zebrafish. Assists PI in preparation and renewal of animal 
protocols. Complies with all safety and infection control standards 
appropriate to this position. Performs other duties as required.  

MINIMUM QUALIFICATIONS: 

1.  Knowledge/Experience:  Bachelors degree in Biology, Fisheries
Science, or Aquaculture and 1 years relevant research in the field. 
2. Skills:  Knowledge of genetics, cell biology and molecular 
biology. Proficient computer skills.  Management /training skills.  
Ability to interact with departmental and institutional offices.

ABOUT UMMS:

The University of Massachusetts Medical School, In Worcester, 
MA is one of the fastest growing medical campuses in the country 
and has built a reputation as a world-class research institution, 
consistently producing noteworthy advances in clinical and basic 
research. Along with medical education, other endeavors supported 
by UMMS include: 
*	Commonwealth Medicine, which supports various state 
agencies and public initiatives throughout Massachusetts; and 
*	Mass Biologic Laboratories in Jamaica Plain, manufacturers of 
vaccines, monoclonal antibodies and plasma products.  

UMMS is an equal opportunity employer and values diversity in the 
workplace. We offer highly competitive salaries based on 
experience; an excellent benefit package including generous time-
off policies, 13 paid holidays, paid continuing education benefits for 
employees and family members, health and dental coverage and a 
great New England location.   UMMS requires the satisfactory 
completion of Employment, Education and Criminal Record 
Reference. UMMS does not accept unsolicited Agency resumes 
and will not pay fees to any 3rd party agency or firm that does not 
have a signed "UMMS Agency Fee Agreement".  

TO APPLY:
Reference Job Code: RP-4GFE/ZF
Email resumes and salary requirements to 
robin.pollier at umassmed.edu,
fax to 508-856-2390 or mail to: University of Massachusetts Medical
School, Human Resources, 419 Belmont Street, Worcester MA 
01604.   

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                            RESEARCH ASSOCIATE #2

The Division of Gene Function and Expression, at the University of 
Massachusetts Medical School, is currently recruiting for a 
researcher to perform a variety of laboratory experiments on 
zebrafish embryos to study molecular processes that govern 
embryonic blood vessel development.  

MAJOR RESPONSIBILITIES: 
Responsible for managing everyday aspects of the lab, including, 
but not limited to: ordering, inventory, maintaing stock reagents, 
maintanence and backup of laboratory databases. Perform 
experiments associated with the genetic characterization of mutant 
and transgenic zebrafish lines. Isolate and purify DNA from 
zebrafish embryos and finclips. Isolate large insert constructs from 
genomic libraries using radiolabeled DNA probes. Perform 
polymerase chain reaction (PCR). Perform Northern and Southern 
blot hybridization using radiolabled probes. Perform whole mount in 
situ hybridization of RNA probes to zebrafish embryos. Sequence 
purified DNA. Performs subcloning and other standard recombinant 
DNA procedures as required. Performs immunoprecipitation and 
Western blot analysis using zebrafish embryo extracts. 
Responsible for managing lab in compliance with institutional 
regulations (radiation safety, chemical safety).  

MINIMUM QUALIFICATIONS: 

1. Knowledge/Experience:  Equivalent to a Bachelors degree in 
Biology and 1 year full time relevant research in the field. 
2. Skills:  Knowledge of basic genetics, cell biology and molecular 
biology.  Proficient computer skills.  Management/training skills.  
Ability to interact with departmental and institutional offices. 
3. Physical Requirements:  Manual dexterity to handle potentially 
hazardous chemicals and/or cleaners. Visual acuity to perform 
duties safely in a scientific laboratory.  

ABOUT UMMS:

The University of Massachusetts Medical School, In Worcester, 
MA is one of the fastest growing medical campuses in the country 
and has built a reputation as a world-class research institution, 
consistently producing noteworthy advances in clinical and basic 
research. Along with medical education, other endeavors supported 
by UMMS include: 

*	Commonwealth Medicine, which supports various state 
agencies and public initiatives throughout Massachusetts; and 
*	Mass Biologic Laboratories in Jamaica Plain, manufacturers of 
vaccines, monoclonal antibodies and plasma products.  

UMMS is an equal opportunity employer and values diversity in the 
workplace.   We offer highly competitive salaries based on 
experience; an excellent benefit package including generous time-
off policies, 13 paid holidays, paid continuing education benefits for 
employees and family members, health and dental coverage and a 
great New England location.  UMMS requires the satisfactory 
completion of Employment, Education and Criminal Record 
Reference. UMMS does not accept unsolicited Agency resumes 
and will not pay fees to any 3rd party agency or firm that does not 
have a signed "UMMS Agency Fee Agreement".  

TO APPLY:
Reference Job Code: RP-5GFE/ZF
Email resumes and salary requirements to 
robin.pollier at umassmed.edu, fax to 508-856-2390 or mail to: 
University of Massachusetts Medical School, Human Resources, 
419 Belmont Street, Worcester MA 01604.  
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Robin Pollier
Human Resources
University of Massachusetts Medical School
419 Belmont Street
Worcester MA 01604
508-856-2185 (office)
508-856-2390 (fax)
For a list of openings: www.umassmed.edu 
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