Polyclonal antiserum in whole mount
Vayuputra
vayuputra369 at hotmail.com
Fri Apr 4 16:35:44 EST 2003
I have obtained some polyclonal (from rabbit) antiserum against a
fusion protein I generated; this antiserum works quite well on western
blots. I was wondering if anyone has suggestions for getting optimal
results using the same antiserum in whole mount
immunohistochemistry on 18-36h fish embryos. I tried it once without
any success. I would greatly appreciate it if anyone can give me some
pointers.
Thanks.
vAyuputra
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