beta-actin-GFP construct

[sam thornton]

Dear Colleagues,

I have been trying to generate a transgenic zebrafish line that 
expresses a Ca2+ reporter protein under the control of a ubiquitous 
promoter. Tragically I have failed at the first hurdle as I have been 
unable to obtain a suitable promoter construct. I have written to a 
number of people who have published suitable promoter elements 
(such as the beta actin or eF1alpha promoters) but have had no 
response. Can any one help??  

If you have a promoter that can be used to drive ubiquitous expression 
from early stages of development (mid-gastrula onwards) and would 
be prepared to let me have some, I'd greatly appreciate hearing from 
you.  

Yours sincerely,

Samantha Thornton

Email - badsam at hotmail.com