Graduate student position

Arindam Majumdar Arindam.Majumdar at
Thu Feb 5 14:08:47 EST 2004

Graduate Student 
(Karolinska Institutet) 

Highly motivated individuals are invited to apply for a graduate student  
appointment in the Division of Matrix Biology (MBB) at the Karolinska  
Institute, Stockholm, Sweden.  Our research group is interested in the  
developmental signals that control growth and differentiation during  
vertebrate kidney organogenesis.  Because the nephron is the  
functional unit of the kidney and the pathophysiological site of human  
kidney diseases, including glomerular pathologies, we have  
concentrated our research efforts on identifying the molecular/genetic  
regulation of nephron formation. Topics of investigation include  
nephron segmentation, tubulogenesis, and glomerular morphogenesis.  
Our lab is using the zebrafish embryonic kidney or pronephros as a  
model system for investigating kidney organogenesis due to several  
experimental advantages including anatomical simplicity, rapid  
development, and the ease plus detail of visualization using molecular  
probes. The zebrafish pronephros system is amenable to genetic  
analysis, and mutants have been informative for identifying important  
genes and cellular interactions during pronephric organogenesis  
(Development, 125, pp. 4655-67, 1998; Dev Gen., 24, pp. 220-29,  
1999; Dev. Biol. 222, pp. 147-57, 2000).  The zebrafish mutant no  
isthmus (noi) contains a mutation in the transcription factor Pax2.1 and  
is associated with pleiotrophic defects including those affecting  
pronephric development.  During pronephric organogenesis,  
pronephric tubules do not form in noi mutants due to problems in  
patterning of the nephric primordium (Development, 127, pp. 2089-98,  
2000).  We are using the noi mutant as an entrance point into  
investigating the genetic pathways that cooperate with Pax2.1 to  
regulate nephron segmentation and renal tubular differentiation.  In  
related projects, we are using the zebrafish pronephric system to study  
the gene function in glomerular maturation and function.  Experimental  
approaches involve genetic screens, antisense morpholino technology,  
microarrays, and transgenesis.   

Research will be conducted in the new Karolinska Institutet Zebrafish  
Facility and   the Division of Matrix Biology, both located in the  
Scheelelaboratoriet on the Karolinska Institutet (Solna) campus.   
Located close to the Stockholm city center, the Karolinska Institutet is  
one of Europe's largest medical universities and has strong and  
dynamic graduate student research programs in the basic and clinical  

Research will be conducted in pursuit of a doctoral degree in  
Molecular, Cell, and Developmental Biology.  KI provides an  
international environment encouraging collaboration and scientific  
interaction.  Interested applicants should have an interest in kidney  
organogenesis and prior research experience. Please send complete  
applications including cover letter, CV, and contact information for  
three references to:  

Arindam Majumdar, PhD  
Division of Matrix Biology  
Deptartment of Medical Biochemistry and Biophysics  
Karolinska Institutet  
Scheeles v=E4g 2  
SE-171 77 Stockholm  
Email: Arindam.Majumdar at   

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